POLK CLIA Kit for Human(Homo sapiens) | EIAab.comPOLK CLIA Kit for Human(Homo sapiens)

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POLK (Gene name),

DNA polymerase kappa (Protein name ), POLK_HUMAN from NCBI database.

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General Annotation

Antigen Annotation

Predicted Eptitope

Vaild Sequence

Related Databases

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Gene name:

POLK(DINB1);

Protein name:

DNA polymerase kappa;

Alternative:

DINB protein(DINP);

Organism:

Human (Homo sapiens).

General Annotation

Sub Unit:

Interacts with REV1 (By similarity). Interacts with PCNA.

Function:

DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity.

Subcellular Location:

Nucleus Detected throughout the nucleus and at replication foci.

Protein Attributes:

Sequence length:

870

Sequence:

50:

100:

150:

200:

250:

300:

350:

400:

450:

500:

550:

600:

650:

700:

750:

800:

850:

870:

STSKKIKPNN | PKHTLDIFFK |

3D Structure:

N/A

Predicted Eptitope:

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Vaild Sequence:

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Related Databases

UniGene:

Hs.135756

String:

Q9UBT6

MIM:

605650

KEGG:

hsa:51426

SMR:

Q9UBT6

Pfam:

PF00817

UniGene:

Hs.726312

Uniprot:

Q9UBT6

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ELISA Kit for Human DNA polymerase kappa

Cat.:

E12062h

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96T

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ELISA Kit for Human DNA polymerase kappa

Cat.:

E12062m

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Manual:

Looking for manual.

MSDS:

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96T

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CLIA Kit for Human DNA polymerase kappa

Cat.:

U12062h

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MSDS:

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Packing:

96T

CLIA Kit for Human DNA polymerase kappa

Cat.:

U12062m

Price:

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MSDS:

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Packing:

96T

Polyclonal Antibody for Human DNA polymerase kappa

Cat.:

P12062Rb-m

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Packing:

40ug/0.2ml

Polyclonal Antibody for Human DNA polymerase kappa

Cat.:

P12062Rb-h

Price:

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Packing:

40ug/0.2ml

Monoclonal Antibody for Human DNA polymerase kappa

Protein for Human DNA polymerase kappa

R&D Technical Data

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Precision

For more information, please refer to the manual,Or contact our technical support: tech@eiaab.com.

Recovery

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Linearity

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References

1.

"Mutation enhancement by DINB1, a mammalian homologue of the Escherichia coli mutagenesis protein dinB."
Ogi T. , Kato T. Jr. , Kato R. , Ohmori H.
Genes Cells4:607-618(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);FUNCTION;TISSUE SPECIFICITY
tissue: Testis.
2.

"Human and mouse homologs of Escherichia coli DinB (DNA polymerase IV), members of the UmuC/DinB superfamily."
Gerlach V.L. , Aravind L. , Gotway G. , Schultz R.A. , Koonin E.V. , Friedberg E.C.
Proc. Natl. Acad. Sci. U.S.A.96:11922-11927(1999) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1);ALTERNATIVE SPLICING;TISSUE SPECIFICITY
tissue: Cervix carcinoma.
3.

"Multiple PolK (POLK) transcripts in mammalian testis."
Guo C. , Gao T. , Confer N. , Velasco-Miguel S. , Friedberg E.C.
DNA Repair4:397-402(2005) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5; 6; 7 AND 8);ALTERNATIVE SPLICING
tissue: Testis.
4.

"Homo sapiens DINP protein mRNA, complete cds."
Poltoratsky V.P. , Scharff M.D.
Submitted (1999-10) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1)
5.

"A bidirectional promoter for the genes encoding DNA polymerase kappa and Goodpasture autoantigen binding protein: identification of a novel pol kappa alternative spliced variant."
Revert-Ros F. , Saus J.
Submitted (2000-11) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 3)
6.

"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T. , Suzuki Y. , Nishikawa T. , Otsuki T. , Sugiyama T. , Irie R. , Wakamatsu A. , Hayashi K. , Sato H. , Nagai K. , Kimura K. , Makita H. , Sekine M. , Obayashi M. , Nishi T. , Shibahara T. , Tanaka T. , Ishii S. , more...
Nat. Genet.36:40-45(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 4)
tissue: Brain.
tissue: Trachea.
7.

NIEHS SNPs program
Submitted (2003-04) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA];VARIANTS ILE-595 AND ASN-832
8.

"The DNA sequence and comparative analysis of human chromosome 5."
Schmutz J. , Martin J. , Terry A. , Couronne O. , Grimwood J. , Lowry S. , Gordon L.A. , Scott D. , Xie G. , Huang W. , Hellsten U. , Tran-Gyamfi M. , She X. , Prabhakar S. , Aerts A. , Altherr M. , Bajorek E. , Black S. , more...
Nature431:268-274(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
9.

Mural R.J. , Istrail S. , Sutton G.G. , Florea L. , Halpern A.L. , Mobarry C.M. , Lippert R. , Walenz B. , Shatkay H. , Dew I. , Miller J.R. , Flanigan M.J. , Edwards N.J. , Bolanos R. , Fasulo D. , Halldorsson B.V. , Hannenhalli S. , Turner R. , more...
Submitted (2005-07) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]
10.

"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res.14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-313 (ISOFORMS 1 AND 2)
tissue: Lymph.
tissue: Spleen.
11.

"Homo sapiens genomic sequence, containing DINB1 gene."
Ogi T. , Yamamoto Y. , Ohmori H.
Submitted (2000-01) to the EMBL/GenBank/DDBJ databases

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-231
12.

"Purification and characterization of pol kappa, a DNA polymerase encoded by the human DINB1 gene."
Gerlach V.L. , Feaver W.J. , Fischhaber P.L. , Friedberg E.C.
J. Biol. Chem.276:92-98(2001) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;COFACTOR;MUTAGENESIS OF ASP-198 AND GLU-199
13.

"Human DNA polymerase kappa bypasses and extends beyond thymine glycols during translesion synthesis in vitro, preferentially incorporating correct nucleotides."
Fischhaber P.L. , Gerlach V.L. , Feaver W.J. , Hatahet Z. , Wallace S.S. , Friedberg E.C.
J. Biol. Chem.277:37604-37611(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
14.

"Localisation of human DNA polymerase kappa to replication foci."
Bergoglio V. , Bavoux C. , Verbiest V. , Hoffmann J.-S. , Cazaux C.
J. Cell Sci.115:4413-4418(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: SUBCELLULAR LOCATION
15.

"Stimulation of DNA synthesis activity of human DNA polymerase kappa by PCNA."
Haracska L. , Unk I. , Johnson R.E. , Phillips B.B. , Hurwitz J. , Prakash L. , Prakash S.
Mol. Cell. Biol.22:784-791(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: INTERACTION WITH PCNA
16.

"Role of human DNA polymerase kappa as an extender in translesion synthesis."
Haracska L. , Prakash L. , Prakash S.
Proc. Natl. Acad. Sci. U.S.A.99:16000-16005(2002) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
17.

"Human DNA polymerase kappa uses template-primer misalignment as a novel means for extending mispaired termini and for generating single-base deletions."
Wolfle W.T. , Washington M.T. , Prakash L. , Prakash S.
Genes Dev.17:2191-2199(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
18.

"A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair."
Haracska L. , Prakash L. , Prakash S.
Genes Dev.17:2777-2785(2003) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION;SCHIFF BASE FORMATION
19.

"Translesion synthesis past 2'-deoxyxanthosine, a nitric oxide-derived DNA adduct, by mammalian DNA polymerases."
Yasui M. , Suzuki N. , Miller H. , Matsuda T. , Matsui S. , Shibutani S.
J. Mol. Biol.344:665-674(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: FUNCTION
20.

"Crystal structure of the catalytic core of human DNA polymerase kappa."
Uljon S.N. , Johnson R.E. , Edwards T.A. , Prakash S. , Prakash L. , Aggarwal A.K.
Structure12:1395-1404(2004) [PubMed] [Europe PMC] [Abstract]

[15/1/25 17:38] Upload to ab completed in less than a minute: 1 file transferred (13.4 Kb/s) Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 68-526

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